His-Tag Protein elisa kit product blog
Tags: ELISA Kit; HIS-TAG PROTEIN; His-Tag Protein elisa kit;
The His-Tag Protein n/a (Catalog #MBS168080) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the His-Tag Protein ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The unknown His-tag samples or recombinant His-tag protein standards are first added to a polyhistidine coated plate. After a brief incubation, an anti-6xHis monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The His-tag protein content in unknown samples is determined by comparing with a standard curve that is prepared from predetermined His-tag protein standards.
Background/Introduction: A polyhistidine-tag, or His-tag, is an amino acid sequence in proteins that consists of at least five histidine (His) residues, usually inserted at the N- or C-terminus of the protein. Often used for affinity purfication of recombinant proteins in E. coli and other prokaryotic or eukaryotic expression systems, the 6xHis-tag motif provides a powerful purification tool while minimizing any effect on the protein\'s functionality and bioactivity. These attributes make it a popular tag for recombinant protein production and isolation. However, the accurate quantitation of His-tagged protein in unpurified samples remains difficult. His-Tag Protein ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of His-tagged protein samples (C- or N-terminal). The quantity of His-tag in a protein sample is determined by comparing its absorbance with that of a known His-tag protein standard curve (provided in the kit). The kit has detection sensitivity range of 4 ug/mL to 1 ng/mL (10 kDa His-tag protein standard), or 400 nM - 100 pM 6xHis-tag residues. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and His-tag samples. The His-Tag Protein ELISA Kit is intended for research use only, and not for test applications.
His-Tag Protein ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.