|The Rat HIF-1alpha hif1a (Catalog #MBS764727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS764727 ELISA Kit recognizes Rat (Rattus, General) HIF-1alpha.
The HIF-1alpha hif1a product has the following accession number(s) (GI #340545531) (NCBI Accession #NP_001230013.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Hypoxia-inducible factor 1-alpha ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Standard Curve
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HIF-1alphaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HIF-1alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF-1alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF-1alphacan be calculated.
Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 7.813-500pg/ml
Sensitivity: 4.688pg/ml. Rat Hypoxia-inducible factor 1-alpha ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. HIF-1alpha also interacts with the following gene(s): ARNT, CREBBP, EGLN1, EGLN3, EP300, HIF1AN, HSP90AA1, TCEB1, UBC, VHL. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to Rat Hypoxia-inducible factor 1-alpha ELISA Kit.