|The Rat HIF-1alpha hif1a (Catalog #MBS764727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS764727 ELISA Kit recognizes Rat (Rattus, General) HIF-1alpha.
The HIF-1alpha hif1a product has the following accession number(s) (GI #340545531) (NCBI Accession #NP_001230013.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Hypoxia-inducible factor 1-alpha ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HIF-1? antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HIF-1? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF-1? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF-1? can be calculated.
Samples: Serum, plasma, tissue homogenates and other biological fluids
Detection Range: 7.813-500pg/ml
Sensitivity: <4.688pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF- 1? were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF- 1? were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Rat Hypoxia-inducible factor 1-alpha ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Vascular tissues are correlated with this protein. HIF-1alpha also interacts with the following gene(s): ARNT, CREBBP, EGLN1, EGLN3, EP300, HIF1AN, HSP90AA1, TCEB1, UBC, VHL. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to Rat Hypoxia-inducible factor 1-alpha ELISA Kit.