|The Human HEPC25 n/a (Catalog #MBS283765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS283765 ELISA Kit recognizes Human (General) HEPC25.
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Intended Uses: For the quantitative detection of Human Hepcidin 25 (HEPC25) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HEPC25 in Human serum, plasma. An antibody specific for HEPC25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEPC25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEPC25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEPC25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 1.56 ng/mL - 100 ng/mL
Sensitivity: 0.78 ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Human Hepcidin 25 (HEPC25) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.