Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow Hepc elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • ACYP2 elisa kit blog
 • tTBK2 elisa kit blog
 • ELMO2 elisa kit blog
 • CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit blog
 • HK2 elisa kit blog
 • KAT2A elisa kit blog
 • IL1zeta elisa kit blog
 • SLC1A5 elisa kit blog
 • ZFAT elisa kit blog
 • TARBP2 elisa kit blog
 • VA elisa kit blog
 • RIOK1 elisa kit blog
 • FRZB elisa kit blog
 • CIDEC / FSP27 elisa kit blog
 • MAP-2 elisa kit blog
 • PANK4 elisa kit blog
 • TAS2R3 elisa kit blog
 • MYO15A elisa kit blog
 • MIP-1beta elisa kit blog
 • IL18RAP elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • TSH Antibody
 • KRT7 - CK7 - Cytokeratin 7 Antibody
 • Angiopoietin 2 (ANG2) ELISA Kit
 • Vascular Endothelial Growth Factor A (VEGF-A) ELISA Kit
 • Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit
 • GPR39 Antibody
 • Adenovirus 36 Antibody (AdV36-Ab) ELISA Kit
 • Insulin Like Growth Factor 2 (IGF2) ELISA Kit
 • Cross Linked C-Telopeptide of Type I Collagen (CTXI) ELISA Kit
 • NEDD8 Antibody
 • Glycoprotein G (G) Recombinant Protein
 • CXCL13 Recombinant Protein
 • HIATL1 Antibody
 • PSMA4 Antibody
 downarrow more ...

Hepc elisa kit product blog

Posted on 2016-06-04 13:06:38 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; Hepcidin; HEPC elisa kit; HEPC;
The Mouse Hepc hamp (Catalog #MBS762097) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762097 ELISA Kit recognizes Mouse (Murine, General) Hepc.

The Hepc hamp product has the following accession number(s) (GI #197097356) (NCBI Accession #NP_001127676.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Hepcidin ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Testing Data Hepc.

Typical Standard Curve
Typical Standard Curve Hepc.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.

Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 125-8000pg/ml
Sensitivity: 75pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepc were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepc were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Mouse Hepcidin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Anemia, Cardiovascular Diseases, Disease Models, Animal, Hemochromatosis, Inflammation, Iron Overload, Kidney Diseases, Liver Diseases, Necrosis, beta-Thalassemia are some of the diseases may be linked to Mouse Hepcidin ELISA Kit. Hepc also interacts with the following gene(s): ATOH8, BMP4, BMP6, CREB3L3, HFE, IL6, SLC11A2, SLC40A1, STAT3.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions