Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow Hepc elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • PAPPA elisa kit blog
 • PARP elisa kit blog
 • AMT elisa kit blog
 • HCRTR1 elisa kit blog
 • NPR2 elisa kit blog
 • GFPT2 elisa kit blog
 • MPI elisa kit blog
 • MANSC1 elisa kit blog
 • HUS1 elisa kit blog
 • PSMD11 elisa kit blog
 • LGALS3BP elisa kit blog
 • PSMD9 elisa kit blog
 • dDAVP elisa kit blog
 • METHYLASE elisa kit blog
 • GDH elisa kit blog
 • SYNGR3 elisa kit blog
 • HSCTN elisa kit blog
 • APOB elisa kit blog
 • HDAC5 elisa kit blog
 • PTHLH elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Lipocalin 5 (LCN5) Antibody
 • IFN-beta ELISA Kit
 • Granzymes A (Gzms-A) ELISA Kit
 • AIM2 Antibody
 • alpha 1-antitrypsin, A1AT Antibody
 • N-Cadherin (Cytoplasmic) (CDH2) Antibody
 • 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase de... Recombinant Protein
 • Heparan Sulfate, Na Salt, Bovine Carbohydrate/Glycoprotein
 • Triggering Receptor Expressed on Myeloid Cells 1 (TREM1) Recombinant Protein
 • Melanocortin receptor 4 (Mc4r) Recombinant Protein
 • Chorionic Gonadotropin beta, Core Fragment Antibody
 • Aquaporin 4 (AQP4) Antibody
 • TBP ELISA Kit
 • Small nuclear ribonucleoprotein Sm D1 (SNRPD1) Recombinant Protein
 downarrow more ...
separator

Hepc elisa kit product blog

separator
Posted on 2016-06-04 13:06:38 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; Hepcidin; HEPC elisa kit; HEPC;
separator
The Mouse Hepc hamp (Catalog #MBS762097) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762097 ELISA Kit recognizes Mouse (Murine, General) Hepc.

The Hepc hamp product has the following accession number(s) (GI #197097356) (NCBI Accession #NP_001127676.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Hepcidin ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Testing Data Hepc.

Typical Standard Curve
Typical Standard Curve Hepc.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.

Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 125-8000pg/ml
Sensitivity: 75pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepc were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepc were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Mouse Hepcidin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Hepc also interacts with the following gene(s): ATOH8, BMP4, BMP6, CREB3L3, HFE, IL6, SLC11A2, SLC40A1, STAT3. Anemia, Cardiovascular Diseases, Disease Models, Animal, Hemochromatosis, Inflammation, Iron Overload, Kidney Diseases, Liver Diseases, Necrosis, beta-Thalassemia are some of the diseases may be linked to Mouse Hepcidin ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions