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HDLC elisa kit product blog

Posted on 2016-04-21 20:17:27 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; HDL-cholesterol; HDLC elisa kit; HDLC;
The Human HDLC n/a (Catalog #MBS288087) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS288087 ELISA Kit recognizes Human (General) HDLC.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

HDLC n/a

Assay Type: Sandwich
Detection Range: 12.5-800 ng/mL
Sensitivity: 8.5ng/mL. Intra-Assay CV: <=3.5%
Inter-Assay CV: <=7.2%
Recovery: 100%. Human HDL-cholesterol ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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