|The Human HDAC6 hdac6 (Catalog #MBS846781) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS846781 Assay Kit recognizes Human (General) HDAC6.
Rapid; high-throughput screening of drugs and novel ligands. ; Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds. Researchers should empirically determine the suitability of the HDAC6 hdac6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HDAC6 hdac6 product has the following accession number(s) (GI #46623327) (NCBI Accession #AAH69243.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Features & Benefits: Simple; highly sensitive; high-throughput compatible ; Rapid screening of HDAC6 inhibitors ; Kit includes Tubacin; an HDAC6 inhibitor; and a stable; recombinant human HDAC6
Applications- • Screening of drugs and novel ligands for interaction with human recombinant HDAC6
Histone Deacetylase 6 (HDAC6; EC 22.214.171.124); is a class IIb HDAC enzyme that deacetylates an epsilon-N-acetyl lysine of histone and non-histone protein substrates. It is an unique member of HDAC family as it contains two deacetylase domains that are proposed to function independently of each other. HDAC6 can shuttle between the nucleus and cytoplasm; suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules. Elevated HDAC6 activity is associated with cell motility and catalyzes increases alpha-tubulin deacetylation; thus influencing cancer cell metastasis. In addition; mutations in HDAC6 gene have been associated with Alzheimer\'s disease. HDAC6-selective inhibitors are considered as promising targets against autoimmune; inflammatory and oncology diseases such as breast and ovarian cancer. HDAC6 Inhibitor Screening Kit utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore; which can be easily quantified using a conventional microplate reader. In the presence of an HDAC6-selective inhibitor (Tubacin); the deacetylase activity of HDAC6 is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HDAC6.
Assay Type: Fluorescence (Ex/Em 380/490 nm)
Samples: Samples containing drugs, inhibitors or ligands (compounds that can interact and affect HDAC6 activity). Adenocarcinoma, Breast Neoplasms, Disease Models, Animal, Fibrosis, Inflammation, Lymphoma, Multiple Myeloma, Neoplasms, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to HDAC6 Inhibitor Screening Kit (Fluorometric). The following patways have been known to be associated with this gene. Blood, Bone, Brain, Heart, Kidney, Lung, Muscle, Prostate, Skin, Thyroid tissues are correlated with this protein.