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HDAC3 assay kit product blog

Posted on 2015-11-21 16:12:05 by mybiosource_staff
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Tags: HDAC3; Assay Kit; HDAC3 assay kit;
The HDAC3 hdac3 (Catalog #MBS841771) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.

The HDAC3 hdac3 product has the following accession number(s) (GI #13128862) (NCBI Accession #NP_003874.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the HDAC3 Inhibitor Screening Kit (Fluorometric) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HDAC3.

Product Information: HDAC3 Inhibitor Screening Kit: High-Throughput Adaptable Fluorometric Assay to Screen HDAC3 Inhibitors. Rapid, Reliable, Simple & Sensitive. Includes Inhibitor Control, Trichostatin A. 100 Assays.

Background: Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In MyBioSource\'s HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.

Summary: Detection method- Fluorescence (Em/Ex = 380/500 nm)
Applications- Suitable for high throughput screening of HDAC3 inhibitors.

Samples: Testing candidate HDAC3 inhibitors. Features & Benefits: Simple procedure; takes ~ 60 min
Fast and convenient
Kit contains the necessary reagents for testing putative HDAC3 inhibitors. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Thyroid tissues are correlated with this protein. HDAC3 also interacts with the following gene(s): EP300, HDAC4, HDAC5, NCOR1, NCOR2, RBBP4, RELA, TBL1X, TBL1XR1, YY1. Adenocarcinoma, Breast Neoplasms, Carcinoma, Hepatocellular, Inflammation, Kidney Diseases, Liver Neoplasms, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to HDAC3 Inhibitor Screening Kit (Fluorometric).
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