HBsAg elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; HBSAG elisa kit; HBSAG; Hepatitis B;
The Human HBsAg n/a (Catalog #MBS284496) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS284496 ELISA Kit recognizes Human HBsAg.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the qualitative detection of Human Hepatitis B virus surface antigen (HBsAg) concentration in serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with HBsAb. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HBsAb and incubated. Then substrate solutions are added to each well. Only those wells that contain HBsAg and enzyme-conjugated HBsAb will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.
Samples: Serum, plasma and other biological fluids. Human Hepatitis B virus surface antigen (HBsAg) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.