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HBF elisa kit product blog

Posted on 2016-10-15 16:59:13 by mybiosource_staff
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Tags: ELISA Kit; Porcine ELISA Kit; HBF elisa kit; HBF;
The Porcine HBF n/a (Catalog #MBS2510930) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2510930 ELISA Kit recognizes Porcine (Pig, Swine, General) HBF.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine HBF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HBF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HBF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HBF. You can calculate the concentration of HBF in the samples by comparing the OD of the samples to the standard curve.

HBF n/a

Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 12.5-800ng/mL
Sensitivity: 7.5ng/mL. Porcine HBF (Fetal Hemoglobin) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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