Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow HB-EGF elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • ZNF397OS elisa kit blog
 • DON elisa kit blog
 • E1/UBAE elisa kit blog
 • C19orf70 elisa kit blog
 • RPL24 elisa kit blog
 • S100A9 elisa kit blog
 • CRESOL elisa kit blog
 • PINP elisa kit blog
 • CROP elisa kit blog
 • DBNDD2 elisa kit blog
 • RHOA elisa kit blog
 • ACTalpha2 elisa kit blog
 • HP-IgM elisa kit blog
 • TLL1 elisa kit blog
 • GHRP elisa kit blog
 • TLX1 elisa kit blog
 • PIGR elisa kit blog
 • VCY elisa kit blog
 • WARS2 elisa kit blog
 • PTOV1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Dog IgM ELISA Kit
 • TRIM34 Antibody
 • ATAD3B Antibody
 • Human Chorionic Gonadotropin (hCG) alpha Antibody
 • BSA (FL11) (BSA-HRP) Antibody
 • pan ADH (ADH1A, B, C) Peptide
 • Hydrophobin (rodA) Recombinant Protein
 • Stromal Cell Derived Factor 1 (SDF1) ELISA Kit
 • Troponin I-Cardiac, Phosphorylated (TNNI3) Antibody
 • fibronectin IgG fraction, biotin labeled (FN1) Antibody
 • anti-macrophage antibody (MSR1) ELISA Kit
 • Interleukin 23R (IL23A) Antibody
 • Catenin delta-2 (Ctnnd2) Recombinant Protein
 • Adenylate kinase 7 (Ak7) Recombinant Protein
 downarrow more ...
separator

HB-EGF elisa kit product blog

separator
Posted on 2016-04-24 20:50:30 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Canine ELISA Kit; Heparin-Binding Epidermal Growth Factor Like-Growth Factor; HB-EGF elisa kit; HB-EGF;
separator
The Canine HB-EGF hbegf (Catalog #MBS739705) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739705 ELISA Kit recognizes Canine (Dog, General) HB-EGF.

The HB-EGF hbegf product has the following accession number(s) (GI #4761593) (NCBI Accession #AAD29416.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HB-EGF.

Principle of the Assay: HB-EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HB-EGF antibody and an HB-EGF-HRP conjugate. The assay sample and buffer are incubated together with HB-EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HB-EGF concentration since HB-EGF from samples and HB-EGF-HRP conjugate compete for the anti-HB-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HB-EGF from the sample, fewer sites are left to bind HB-EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HB-EGF concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 50-1000pg/mL
Sensitivity: 1.0pg/mL. Intended Uses: This HB-EGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HB-EGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Ovary tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions