Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow Gzms-A elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • GPR126 elisa kit blog
 • TBC1D20 elisa kit blog
 • GPR109A elisa kit blog
 • FPSA elisa kit blog
 • PRAF2 elisa kit blog
 • APP elisa kit blog
 • RHBDF2 elisa kit blog
 • COL-I Ab elisa kit blog
 • Caspase-12 elisa kit blog
 • DDX17 elisa kit blog
 • CXCL2 elisa kit blog
 • KCTD18 elisa kit blog
 • ISM1 elisa kit blog
 • THBS1 elisa kit blog
 • TMEM57 elisa kit blog
 • FLOT1 elisa kit blog
 • REG3G elisa kit blog
 • alpha Manase elisa kit blog
 • IL6R elisa kit blog
 • EGLN1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Muc19-Smgc Antibody
 • LEPTOSPIRA BIFLEXA Antibody
 • heat shock 105kDa-110kDa protein 1 (HSPH1) ELISA Kit
 • Thyroid Hormone Receptor, a2-Isotype (THRA) Antibody
 • Osteoprotegerin (OPG) ELISA Kit
 • JAK2 ELISA Kit
 • Nuclear Mitotic Apparatus Protein (NuMA) (NUMA1) Antibody
 • Insulin-Like Growth Factor 1 (IGF-1) ELISA Kit
 • Proliferating Cell (PCNA) Recombinant Protein
 • Platelet Derived Growth Factor Receptor Beta (PDGFRbeta) ELISA Kit
 • 60S ribosomal protein L38 (RPL38) Antibody
 • Glutathione S-Transferase Omega 1 (GST-01) Western Blot Control
 • MMP-9 ELISA Kit
 • Indian Hedgehog (IHH) Recombinant Protein
 downarrow more ...
separator

Gzms-A elisa kit product blog

separator
Posted on 2015-11-08 08:02:24 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; Granzymes A; Gzms-A elisa kit; Gzms-A;
separator
The Mouse Gzms-A gzma (Catalog #MBS723830) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS723830 ELISA Kit recognizes Mouse (Murine, General) Gzms-A.

The Gzms-A gzma product has the following accession number(s) (GI #5453676) (NCBI Accession #NP_006135.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Granzymes An ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Gzms-A.

Principle of the Assay: GZMS-An ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GZMS-An antibody and an GZMS-A-HRP conjugate. The assay sample and buffer are incubated together with GZMS-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GZMS-A concentration since GZMS-A from samples and GZMS-A-HRP conjugate compete for the anti-GZMS-An antibody binding site. Since the number of sites is limited, as more sites are occupied by GZMS-A from the sample, fewer sites are left to bind GZMS-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GZMS-A concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100ng/mL
Sensitivity: 1.0ng/mL. Intended Uses: This GZMS-An ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse GZMS-A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Mouse Granzymes An ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Brain, Heart, Intestine, Kidney, Lung, Lymph Node, Skin, Spleen, Uterus tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions