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GSH-PX elisa kit product blog

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Posted on 2017-11-27 04:17:44 by mybiosource_staff
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Tags: ELISA Kit; Canine ELISA Kit; Glutathione Peroxidase; GSH-PX elisa kit; GSH-PX;
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The Canine GSH-PX n/a (Catalog #MBS040897) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS040897 ELISA Kit recognizes Canine (Dog, General) GSH-PX.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GSH-PX concentrations in Canine serum, plasma and other body fluids. Using Purified Canine GSH-PX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSH-PX and GSH-PX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSH-PX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

GSH-PX n/a

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type: Sandwich
Detection Range: 15.6 U/L - 500 U/L.
Sensitivity: 2.0 U/L. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GSH-PX concentrations in Canine serum, plasma and other body fluids. Using Purified Canine GSH-PX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSH-PX and GSH-PX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSH-PX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) is less than 15%.
Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]. Canine Glutathione Peroxidase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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