GR-alpha elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Glucocorticoid Receptor-alpha; GR-alpha elisa kit; GR-alpha;
The Rabbit GR-alpha n/a (Catalog #MBS720527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720527 ELISA Kit recognizes Rabbit GR-alpha.The GR-alpha n/a product has the following accession number(s) (GI #38639409) (Uniprot Accession #Q865Y6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit glucocorticoid receptor-alpha ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This GR-An ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GR-An in the sample, this GR-An ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GR-A concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of GR-An utilizes a multiclonal anti-GR-An antibody and an GR-A-HRP conjugate. The assay sample and buffer are incubated together with GR-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GR-A concentration since GR-A from samples and GR-A-HRP conjugate compete for the anti-GR-An antibody binding site. Since the number of sites is limited, as more sites are occupied by GR-A from the sample, fewer sites are left to bind GR-A-HRP conjugate. Standards of known GR-A concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of GR-A. The GR-A concentration in each sample is interpolated from this standard curve.
Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Rabbit glucocorticoid receptor-alpha ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.