|The Human GPR179 gpr179 (Catalog #MBS280907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS280907 ELISA Kit recognizes Human (General) GPR179.
The GPR179 gpr179 product has the following accession number(s) (GI #629266054) (NCBI Accession #NP_001004334.3) (Uniprot Accession #Q6PRD1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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GPR179, Belongs to the G-protein coupled receptor 3 family. G protein-coupled receptors (GPCRs), comprise a large protein family of transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses.
The class C G-protein-coupled receptors are a class of G-protein coupled receptors that include the metabotropic glutamate receptors and several additional receptors. Family C GPCRs have a large extracellular N-terminus which binds the orthosteric (endogenous) ligand. The shape of this domain is often likened to a clam. Several allosteric ligands to these receptors have been identified and these bind within the seven transmembrane region.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR179 in samples. An antibody specific for GPR179 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR179 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR179 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR179 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Human Probable G-protein coupled receptor 179 (GPR179) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.