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GM1b elisa kit product blog

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Posted on 2015-10-30 02:08:33 by mybiosource_staff
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Tags: ELISA Kit; Sheep ELISA Kit; Anti-Ganglioside M1b Antibody; GM1b elisa kit; GM1b;
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The Sheep GM1b n/a (Catalog #MBS745873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS745873 ELISA Kit recognizes Sheep (Ovine, General) GM1b.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Anti-Ganglioside M1b Antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) GM1b.

Intended Uses: This Anti-GM1b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Anti-GM1b. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Anti-GM1b ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside M1b antigen and an Anti-GM1b-HRP conjugate. The assay sample and buffer are incubated together with Anti-GM1b-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GM1b concentration since Anti-GM1b from samples and Anti-GM1b-HRP conjugate compete for the Ganglioside M1b antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-GM1b from the sample, fewer sites are left to bind Anti-GM1b-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GM1b concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Sheep Anti-Ganglioside M1b Antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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