Glycine elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Glycine; Glycine elisa kit;
The Human Glycine n/a (Catalog #MBS495083) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS495083 ELISA Kit recognizes Human Glycine.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Glycine ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: Enzyme Immunoassay for the determination of free Metanephrine and free Normetanephrine in plasma. For research use only.
Principle of the Assay: Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards
Background/Introduction: Metanephrine and Normetanephrine are the metabolites of the catecholamines Epinephrine and Norepinephrine, respectively. Cells derived from neuroendocrine tumors (e.g. pheochromcytoma) are known to produce catecholamines which are secreted episodically via vesicles into the blood stream. But beside this a small portion of the catecholamines is metabolized inside the cells to the corresponding catecholamines metabolites-namely Metanephrine, Normetanephrine and 3-Methoxytyramine-which are secreted at low levels continuously into the blood stream.
Samples: Plasma
Assay Type: Competitive. Glycine ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.