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GJA8 elisa kit product blog

Posted on 2017-04-16 17:46:38 by mybiosource_staff
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Tags: ELISA Kit; Sheep ELISA Kit; GJA8; GJA8 elisa kit; Gap junction alpha-8;
The Sheep GJA8 gja8 (Catalog #MBS285084) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS285084 ELISA Kit recognizes Sheep (Ovine, General) GJA8.

The GJA8 gja8 product has the following accession number(s) (GI #194239653) (NCBI Accession #NP_001123510.1) (Uniprot Accession #P55917). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

GJa8 encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome.Defects in GJA8 are the cause of cataract-microcornea syndrome. Cataract-microcornea syndrome is characterized by the association of congenital cataract and microcornea without any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most often bilateral posterior polar with opacification in the lens periphery.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GJA8 in samples. An antibody specific for GJA8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGJA8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GJA8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GJA8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

GJA8 gja8

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Sheep Gap junction alpha-8 protein (GJA8) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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