GHRP-Ghrelin elisa kit product blog
Tags: ELISA Kit; Pig ELISA Kit; growth hormone releasing peptide-Ghrelin; GHRP-Ghrelin elisa kit; GHRP-Ghrelin;
The Pig GHRP-Ghrelin n/a (Catalog #MBS281319) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281319 ELISA Kit recognizes Pig GHRP-Ghrelin.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GHRP-Ghrelin in samples. An antibody specific for GHRP-Ghrelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRP-Ghrelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRP-Ghrelin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRP-Ghrelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Pig Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.