GABA elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; GABA elisa kit; GABA; GABA (Gamma-aminobutyric acid);
The Rat GABA gaba (Catalog #MBS7606513) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7606513 ELISA Kit recognizes Rat GABA.The GABA gaba product has the following accession number(s) (GI #300078440) (NCBI Accession #ADJ67161.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat GABA (Gamma-aminobutyric acid) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with GABA. During the reaction, GABA in the sample or standard competes with a fixed amount of GABA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to GABA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABA in the samples is then determined by comparing the O D of the samples to the standard curve.
Samples: serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 31.2-2000pg/ml
Sensitivity: <18.75pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Rat GABA (Gamma-aminobutyric acid) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.