|The Human G6PC g6pc (Catalog #MBS454366) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS454366 ELISA Kit recognizes Human (General) G6PC.
The G6PC g6pc product has the following accession number(s) (GI #393537031) (NCBI Accession #NP_000142.2) (Uniprot Accession #P35575). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of G6PC in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G6PC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to G6PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G6PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of G6PC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Detection Range: 0.156-10ng/mL
Sensitivity: 0.063ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G6PC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G6PC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. G6PC also interacts with the following gene(s): CREB1, FOXO1, HNF1A, PGM1. Kidney, Liver tissues are correlated with this protein. Carcinoma, Hepatocellular, Disease Models, Animal, Fatty Liver, Glycogen Storage Disease Type I, Hepatomegaly, Hepatorenal form of glycogen storage disease, Hyperglycemia, Inflammation, Insulin Resistance, Liver Diseases are some of the diseases may be linked to Human Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit.