Fumonisin B1 elisa kit product blog
Tags: ELISA Kit; Fumonisin B1; Fumonisin B1 elisa kit;
The Fumonisin B1 n/a (Catalog #MBS282500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Fumonisin B1 (FB1) concentration in corn, feed and cooking oil.
Principle of the Assay: This assay is based on the competitive enzyme immunoassay for the detection of FB1 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The FB1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-FB1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the FB1 in it. This value is compared to the standard curve and the FB1 concentration is subsequently obtained.
Samples: Corn, feed and cooking oil
Assay Type: Quantitative Competitive
Detection Range: 0.5 ppb - 40.5 ppb
Sensitivity: 0.5 ppb. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Fumonisin B1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.