|The Pig FT4 ft4 (Catalog #MBS936406) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS936406 ELISA Kit recognizes Pig (Porcine, Swine, General) FT4.
The FT4 ft4 product has the following accession number(s) (GI #309259079) (NCBI Accession #ADO61927.1) (Uniprot Accession #E3TSA0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig free thyroxine, FT4 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FT4. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated FT4. A competitive inhibition reaction is launched between FT4 (Standards or samples) and Biotin-conjugated FT4 with the pre-coated antibody specific for FT4. The more amount of FT4 in samples, the less antibody bound by Biotin-conjugated FT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, cell culture supernates, tissue homogenates
Assay Type: Competitive
Detection Range: 4 pmol/L-64 pmol/L
Sensitivity: 2.151 pmol/L. Detection Wavelength: 450nm
Sample Volume: 50-100ul
Protein Biological Process 1: Thyroid function. Pig free thyroxine, FT4 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. FT4 also interacts with the following gene(s): FLNA. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.