fT3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; fT3 elisa kit; fT3;
The Human fT3 n/a (Catalog #MBS2509611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2509611 ELISA Kit recognizes Human fT3.The fT3 n/a product has the following accession number(s) (GI #555948276) (NCBI Accession #AGZ20209.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human fT3 (Free Triiodothyronine) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human fT3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with fT3. During the reaction, fT3 in the sample or standard competes with a fixed amount of fT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to fT3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, Plasma, Biological Fluids
Assay Type: Competitive
Detection Range: 0.781-50ng/mL
Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL. Human fT3 (Free Triiodothyronine) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.