Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow FSTL1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • S100beta elisa kit blog
 • HB-PS1Ag elisa kit blog
 • YAP1 elisa kit blog
 • ADORA1 elisa kit blog
 • IL-7 elisa kit blog
 • PLPL elisa kit blog
 • LY75 elisa kit blog
 • CDK-7 elisa kit blog
 • EFHA2 elisa kit blog
 • APOC1 elisa kit blog
 • CD302 elisa kit blog
 • CX43 elisa kit blog
 • SERT elisa kit blog
 • C-MYC elisa kit blog
 • alpha2-MG elisa kit blog
 • EMA IgA elisa kit blog
 • RXRgamma elisa kit blog
 • CYFRA21-1 elisa kit blog
 • BCKDHA elisa kit blog
 • NPFF elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • ADIPOR1 Antibody
 • protein phosphatase 1, regulatory (inhibitor) subunit 15B (P... ELISA Kit
 • Chemokine (C-X-C motif) ligand 10 (CXCL10-IP10) ELISA Kit
 • Neurokinin A (NKA) ELISA Kit
 • Virus-like Particles (VLP) Recombinant Protein
 • hemopoietic cell kinase (HCK) Antibody
 • Asporin-ASPN Antibody
 • Alpha 1-Antitrypsin Reference Standard (SERPINA1) Reference Serum & Calibrator
 • CIDE-A Antibody
 • TENM3 Antibody
 • 12-Lipoxygenase (LOX-12) ELISA Kit
 • Beta-lactamase OXA-9 (bla) Recombinant Protein
 • Ferritin ELISA Kit
 • Gzms-A ELISA Kit
 downarrow more ...
separator

FSTL1 elisa kit product blog

separator
Posted on 2017-08-05 05:24:07 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; FSTL1; FSTL1 elisa kit;
separator
The Human FSTL1 fstl1 (Catalog #MBS177300) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS177300 ELISA Kit recognizes Human (General) FSTL1.

The FSTL1 fstl1 product has the following accession number(s) (GI #2498390) (NCBI Accession #Q12841.1) (Uniprot Accession #Q12841). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human FSTL1 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human FSTL1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only) FSTL1.

Principle of the assay: human FSTL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been precoated onto 96-well plates. Standards(NSO, M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FSTL1 amount of sample captured in plate.
Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 312pg/ml-20,000pg/ml
Sensitivity: < 10pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human FSTL1 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions