|The Mouse FGL1 fgl1 (Catalog #MBS903221) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS903221 ELISA Kit recognizes Mouse (Murine, General) FGL1.
The FGL1 fgl1 product has the following accession number(s) (GI #262331536) (NCBI Accession #NP_663569.2) (Uniprot Accession #Q71KU9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Fibrinogen-like protein 1, FGL1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FGL1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for FGL1. The competitive inhibition reaction is launched between with pre-coated FGL1 and FGL1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FGL1 in the samples. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 31.25 pg/ml-2000 pg/ml
Sensitivity: 7.8 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Mouse Fibrinogen-like protein 1, FGL1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Carcinoma, Hepatocellular, Disease Models, Animal, Drug Toxicity, Drug-Induced Liver Injury, Inflammation, Liver Diseases, Liver Failure, Acute, Liver Neoplasms, Necrosis, Neoplasms are some of the diseases may be linked to Mouse Fibrinogen-like protein 1, FGL1 ELISA Kit. Liver, Pancreas tissues are correlated with this protein.