|The Rat FGF7 fgf7 (Catalog #MBS2019274) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2019274 ELISA Kit recognizes Rat (Rattus, General) FGF7.
The FGF7 fgf7 product has the following accession number(s) (GI #4503705) (NCBI Accession #NP_002000.1) (Uniprot Accession #P21781). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the ELISA Kit for Fibroblast Growth Factor 7 (FGF7) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FGF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FGF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Sandwich
Detection Range: 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL
Sensitivity: 5.8pg/mL. ELISA Kit for Fibroblast Growth Factor 7 (FGF7) or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Fibrosis, Hyperplasia, Inflammation, Lung Injury, Necrosis, Neoplasms, Experimental, Prostatic Hyperplasia, Pulmonary Fibrosis are some of the diseases may be linked to ELISA Kit for Fibroblast Growth Factor 7 (FGF7). Bone, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Muscle, Prostate, Skin, Vascular tissues are correlated with this protein. FGF7 also interacts with the following gene(s): EGF, FGF1, FGF23, FGF4, FGF6, FGFBP1, FGFR1, FGFR2, FGFR3, FGFR4.