FGF21 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; FGF21; FGF21 elisa kit; Fibroblast Growth Factor 21 (FGF21);
The Mouse FGF21 fgf21 (Catalog #MBS2025113) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2025113 ELISA Kit recognizes Mouse FGF21.The FGF21 fgf21 product has the following accession number(s) (GI #9506597) (NCBI Accession #NP_061986.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF21 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FGF21 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF21 and unlabeled FGF21 (Standards or samples) with the pre-coated antibody specific to FGF21. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF21 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF21 in the sample.
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 12.5-200pg/mL
Sensitivity: 5.2pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF21 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF21 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. FGF21 also interacts with the following gene(s): AKT1, CYP7A1, FGFR1, FGFR2, FGFR3, FGFR4, INS, KLB, PPARG. Cardiovascular Diseases, Diabetes Mellitus, Type 2, Disease Models, Animal, Fatty Liver, Hyperinsulinism, Inflammation, Insulin Resistance, Liver Diseases, Obesity, Weight Loss are some of the diseases may be linked to Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit. Lung tissues are correlated with this protein. The following patways have been known to be associated with this gene.