Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow FBLN1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • HOXB8 elisa kit blog
 • CAM-Ab elisa kit blog
 • NUP35 elisa kit blog
 • NCAM elisa kit blog
 • NSL1 elisa kit blog
 • alpha-La elisa kit blog
 • ILF2 elisa kit blog
 • RNASE elisa kit blog
 • RBM38 elisa kit blog
 • HMGCS elisa kit blog
 • ACTB elisa kit blog
 • DACT2 elisa kit blog
 • FABP12 elisa kit blog
 • RPS15 elisa kit blog
 • AGC elisa kit blog
 • PPARgamma elisa kit blog
 • BGN elisa kit blog
 • ZP1 elisa kit blog
 • HPRT1 elisa kit blog
 • DR1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Tubulin Antibody
 • ZAP 70 Antibody
 • Orexin A (OXA) ELISA Kit
 • Chorionic Gonadotropin Beta Polypeptide (CGbeta) ELISA Kit
 • STAT2 Antibody
 • SOX2 Antibody
 • APOE Peptide
 • Interleukin 6 (IL-6) ELISA Kit
 • L-lactate dehydrogenase A chain (LDHA) Recombinant Protein
 • ZSCAN29 cDNA Clone
 • sCD105-soluble Endoglin ELISA Kit
 • FDCSP Antibody
 • Serotonin-5 Hydroxytryptamine (5-HT) ELISA Kit
 • THEOPHYLLINE Antibody
 downarrow more ...
separator

FBLN1 elisa kit product blog

separator
Posted on 2017-06-05 11:42:55 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; FBLN1 elisa kit; FBLN1; Fibulin-1;
separator
The Human FBLN1 fbln1 (Catalog #MBS762898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762898 ELISA Kit recognizes Human (General) FBLN1.

The FBLN1 fbln1 product has the following accession number(s) (GI #15822648) (NCBI Accession #AAK82945.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Fibulin-1 ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data FBLN1.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FBLN1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FBLN1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FBLN1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FBLN1 can be calculated.

Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 0.47-30ng/ml
Sensitivity: <0.281ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FBLN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FBLN1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Human Fibulin-1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Embryonic Tissue, Eye, Heart, Kidney, Muscle, Nerve, Prostate, Skin tissues are correlated with this protein. Adenocarcinoma, Brain Diseases, Cell Transformation, Neoplastic, Congenital Abnormalities, Disease Models, Animal, Kidney Diseases, Neoplasms, Experimental, Prostatic Neoplasms, Skin Diseases, Synpolydactyly 2 are some of the diseases may be linked to Human Fibulin-1 ELISA Kit. FBLN1 also interacts with the following gene(s): FBLN5, HSPG2, MFAP5, NID1, VCAN. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions