Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow F12 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • PROC elisa kit blog
 • DDX4 elisa kit blog
 • MC1R elisa kit blog
 • Ki67P elisa kit blog
 • LOX elisa kit blog
 • AFMID elisa kit blog
 • IGFBP-4 elisa kit blog
 • TAT1 elisa kit blog
 • HDL elisa kit blog
 • ADAMTS12 elisa kit blog
 • GLCE elisa kit blog
 • IL1R1 elisa kit blog
 • MYOM1 elisa kit blog
 • ADRb3 elisa kit blog
 • BLYS elisa kit blog
 • SapoVirus (SV) pcr kit blog
 • ZBED5 elisa kit blog
 • CHM elisa kit blog
 • URAT1 elisa kit blog
 • RAGE elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • plasmin-antiplasmin complex, PAP ELISA Kit
 • VAP-1 ELISA Kit
 • UCP1 Antibody
 • alpha Synuclein (SNCA) Antibody
 • MYCBP associated protein (MYCBPAP) Antibody
 • Casein kinase II subunit Alpha (CSNK2A1) ELISA Kit
 • Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit
 • KIAA1191 Antibody
 • Taq Plus DNA Polymerase Recombinant Protein
 • AU1 FITC Antibody
 • glycine amidinotransferase (L-arginine:glycine amidinotransf... ELISA Kit
 • Monosialoganglioside GM4 Ganglioside
 • Alkaline Phosphatase (ALP) Recombinant Protein
 • NOD2 Antibody
 downarrow more ...
separator

F12 elisa kit product blog

separator
Posted on 2016-01-03 21:10:53 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Monkey ELISA Kit; F12; F12 elisa kit; coagulation Factor XII;
separator
The Monkey F12 f12 (Catalog #MBS739542) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739542 ELISA Kit recognizes Monkey (Rhesus Macaque, Rhesus Monkey, General) F12.

The F12 f12 product has the following accession number(s) (GI #4165317) (NCBI Accession #BAA37148.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Monkey Coagulation Factor XII ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) F12.

Principle of the Assay: F12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F12 antibody and an F12-HRP conjugate. The assay sample and buffer are incubated together with F12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F12 concentration since F12 from samples and F12-HRP conjugate compete for the anti-F12 antibody binding site. Since the number of sites is limited, as more sites are occupied by F12 from the sample, fewer sites are left to bind F12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F12 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 5.0-100ng/mL
Sensitivity: 1.0ng/mL. Intended Uses: This F12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey F12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Monkey Coagulation Factor XII ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Carcinoma, Cardiovascular Diseases, Cell Transformation, Neoplastic, Fibrosis, Liver Diseases, Liver Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases, Thrombosis are some of the diseases may be linked to Monkey Coagulation Factor XII ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions