|The F12 f12 (Catalog #MBS135943) is an ELISA Kit produced from Mouse and is intended for research purposes only. The product is available for immediate purchase.
The F12 f12 product has the following accession number(s) (GI #171906584) (NCBI Accession #NP_067464.2) (Uniprot Accession #Q80YC5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation. The sensitive quantitative measurement of total mouse Factor XII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XII and XIIa will be detected by the assay. The concentration of Factor XII in normal human plasma ranges from 15-47 ug/ml. Normal values of Factor XII in mouse plasma have not been conclusively determined but are believed to be similar to human plasma based on in-house testing. The assay measures total mouse Factor XII in the 0.1-100 ng/ml range. Samples giving mouse Factor XII levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:50,000 to 1:400,000 dilution for plasma is suggested for best results. Mouse Factor XII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XII in the samples. A standard calibration curve is prepared using dilutions of purified Factor XII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Source Info: Multiple
Target Info: Factor XII. Mouse Factor XII total antigen assay ELISA kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.