|The Guinea Pig ET-1 edn1 (Catalog #MBS265321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS265321 ELISA Kit recognizes Guinea Pig (Cavia, Cavy, Porcellus, General) ET-1.
The ET-1 edn1 product has the following accession number(s) (GI #556203) (NCBI Accession #AAA52339.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Guinea pig Endothelin 1 (ET-1) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig ET-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Samples: Guinea pig serum, plasma or cell culture supernatant and organizations in the natural and recombinant ET-1 concentration.
Assay Type: Sandwich
Detection Range: 500 pg/ml-7.8 pg/ml
Sensitivity: The minimum detectable Guinea pig ET-1 up to 1 pg/ml. Intra-assay Precision: <= 8%
Inter-assay Precision: <= 12%. Guinea pig Endothelin 1 (ET-1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Asthma, Cardiovascular Abnormalities, Cardiovascular Diseases, Craniofacial Abnormalities, Heart Defects, Congenital, Heart Failure, Hypertension, Inflammation, Kidney Diseases, Necrosis are some of the diseases may be linked to Guinea pig Endothelin 1 (ET-1) ELISA Kit. Brain, Liver tissues are correlated with this protein. The following patways have been known to be associated with this gene. ET-1 also interacts with the following gene(s): AKT1, ECE1, EDNRA, EDNRB, FOS, GATA2, HIF1A, JUN.