|The General Erythromycin N-demethylase n/a (Catalog #MBS8243223) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS8243223 Assay Kit recognizes General (Zebra) Erythromycin N-demethylase. MyBioSource\'s Erythromycin N-demethylase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the Erythromycin N-demethylase n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Background/Introduction: Cytochrome P450 enzymes play an important role in the metabolism of exogenous substrate, especially drugs and poisons. As an important enzyme of P450 family, Erythromycin N-demethylase (ERND) is equivalent to isoform CYP2B and closely related to the demethylation reaction of drugs. CYP2B can catalytic the forming of inactive metabolites that easy be excreted, so that it is a detoxification agent, and it also allows certain drugs be activation by CYP2B metabolic. ERND catalytic erythromycin release formaldehyde, formaldehyde content was measured by a colorimetric Nash, thus ERND activity can be calculated.
Samples: Tissue extracts, Cell lysate Samples.