EPP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; EPP; EPP elisa kit; Erythrocyte Protoporphyrin (EPP);
The Human EPP n/a (Catalog #MBS2000198) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2000198 ELISA Kit recognizes Human EPP.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythrocyte Protoporphyrin (EPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythrocyte Protoporphyrin (EPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythrocyte Protoporphyrin (EPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythrocyte Protoporphyrin (EPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Plasma
Assay Type: Sandwich
Detection Range: 3.125-200 ng/mL The standard curve concentrations used for the ELISA\'s were 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL
Sensitivity: Typically less than 1.2 ng/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythrocyte Protoporphyrin (EPP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythrocyte Protoporphyrin (EPP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
. Human Erythrocyte Protoporphyrin (EPP) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.