Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow EPO elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • Orexins elisa kit blog
 • CIT elisa kit blog
 • LIF elisa kit blog
 • ARL3 elisa kit blog
 • GTF2A1 elisa kit blog
 • UROD elisa kit blog
 • AT1RA elisa kit blog
 • ERCC1 elisa kit blog
 • SPDYE5 elisa kit blog
 • SDC3 elisa kit blog
 • LCM elisa kit blog
 • SHPRH elisa kit blog
 • LTA elisa kit blog
 • P16 elisa kit blog
 • CPB1 elisa kit blog
 • ATAD3C elisa kit blog
 • HCRTR1 elisa kit blog
 • GNE elisa kit blog
 • SOX5 elisa kit blog
 • RPL23A elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • 25-hydroxycholesterol 7-alpha-hydroxylase (Cyp7b1) Recombinant Protein
 • CKII alpha (CSNK2A1) Antibody
 • OPRM1 Antibody
 • Telomerase ELISA Kit
 • musashi homolog 1 (Drosophila) (MSI1) ELISA Kit
 • Chlamydia trachomatis Antibody
 • TFAM Antibody
 • Baculoviral IAP repeat-containing protein 6 (BIRC6) ELISA Kit
 • GFR-1 (IN) Antibody
 • ACOX2 Antibody
 • Glucose Regulated Protein 78 (GRP78) ELISA Kit
 • bFGF Antibody
 • oxidizided glutathione, GSSG ELISA Kit
 downarrow more ...

EPO elisa kit product blog

Posted on 2016-05-13 20:20:13 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Chicken ELISA Kit; Erythropoietin; EPO; EPO elisa kit;
The Chicken EPO epo (Catalog #MBS739710) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739710 ELISA Kit recognizes Chicken (Gallus, General) EPO.

The EPO epo product has the following accession number(s) (GI #62240997) (NCBI Accession #NP_000790.2) (Uniprot Accession #P01588). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Chicken Erythropoietin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) EPO.

Principle of the Assay: EPO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPO antibody and an EPO-HRP conjugate. The assay sample and buffer are incubated together with EPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPO concentration since EPO from samples and EPO-HRP conjugate compete for the anti-EPO antibody binding site. Since the number of sites is limited, as more sites are occupied by EPO from the sample, fewer sites are left to bind EPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPO concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This EPO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken EPO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Chicken Erythropoietin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Anemia, Brain Diseases, Central Nervous System Diseases, Hematologic Diseases, Inflammation, Kidney Diseases, Kidney Failure, Chronic, Neoplasms, Nervous System Diseases, Renal Insufficiency are some of the diseases may be linked to Chicken Erythropoietin ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions