|The Rat ENPP1 enpp1 (Catalog #MBS912067) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS912067 ELISA Kit recognizes Rat (Rattus, General) ENPP1.
The ENPP1 enpp1 product has the following accession number(s) (GI #16758300) (NCBI Accession #NP_445987.1) (Uniprot Accession #Q924C3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENPP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 3.12 ng/ml -200 ng/ml
Sensitivity: 0.78 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, ENPP1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Arterial calcification of infancy, Cardiovascular Diseases, Diabetes Mellitus, Type 2, Fibrosis, Hypertension, Kidney Diseases, Liver Diseases, Myocardial Ischemia, Obesity, Ossification of Posterior Longitudinal Ligament are some of the diseases may be linked to Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, ENPP1 ELISA Kit. The following patways have been known to be associated with this gene. Brain, Colon, Connective Tissue, Embryonic Tissue, Muscle, Ovary, Pancreas, Placenta tissues are correlated with this protein.