Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow ELOA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • ACBD6 elisa kit blog
 • PACAP-27 elisa kit blog
 • UACA elisa kit blog
 • OR2H2 elisa kit blog
 • INHbC elisa kit blog
 • 16-A-OHE-1 elisa kit blog
 • FBN2 elisa kit blog
 • GMFB elisa kit blog
 • PIGR elisa kit blog
 • S100A9 elisa kit blog
 • LN elisa kit blog
 • CORO7 elisa kit blog
 • ATP1alpha1 elisa kit blog
 • DGAT1 elisa kit blog
 • Cholic acid elisa kit blog
 • VE elisa kit blog
 • ALPK1 elisa kit blog
 • BDKRB2 elisa kit blog
 • SPV elisa kit blog
 • sFlt-1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • p27 Kip1 (Phospho-Thr198) Antibody
 • Adrenergic Receptor alpha-2A (ADRA2A) Antibody
 • Epidermal Growth Factor Receptor 2 Extracellular Domain (HER... ELISA Kit
 • Heat Shock Protein 70 (HSP70) ELISA Kit
 • Ultrasensitivity Thyroxine (U-T4) ELISA Kit
 • Advanced Oxidation Protein Products (AOPP) ELISA Kit
 • Acetyl Coenzyme A Carboxylase Alpha (ACACA) ELISA Kit
 • Fibronection Related Antigen (FRA) ELISA Kit
 • Growth-Regulated Oncogeney-Melanoma Growth Stimulating Activ... ELISA Kit
 • Phosphatidyl Ethanol (PEth) ELISA Kit
 • Toll Like Receptor2 (TLR2) ELISA Kit
 • Lymphotactin (LTN) ELISA Kit
 • Protein Kinase K9 (PKK9) ELISA Kit
 • Z-Dna Binding Protein (ZBP1) ELISA Kit
 downarrow more ...
separator

ELOA elisa kit product blog

separator
Posted on 2016-01-31 03:32:33 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Sheep ELISA Kit; Elongin A; ELOA elisa kit; ELOA;
separator
The Sheep ELOA tceb3 (Catalog #MBS744528) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS744528 ELISA Kit recognizes Sheep (Ovine, General) ELOA.

The ELOA tceb3 product has the following accession number(s) (GI #992563). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Elongin An ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) ELOA.

Intended Uses: This ELOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ELOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ELOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELOA antibody and an ELOA-HRP conjugate. The assay sample and buffer are incubated together with ELOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELOA concentration since ELOA from samples and ELOA-HRP conjugate compete for the anti-ELOA antibody binding site. Since the number of sites is limited, as more sites are occupied by ELOA from the sample, fewer sites are left to bind ELOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELOA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Sheep Elongin An ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Testis tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions