|The Mouse EGF epidermal growth factor/ egf (Catalog #MBS701027) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS701027 ELISA Kit recognizes Mouse (Murine, General) EGF.
The EGF epidermal growth factor/ egf product has the following accession number(s) (GI #688072) (NCBI Accession #AAB32226.1) (Uniprot Accession #Q28867). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Epidermal growth factor, EGF ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated antibody preparation specific for EGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates
Detection Range: 0.312 pg/ml-20 pg/ml
Sensitivity: 0.078 pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Mouse Epidermal growth factor, EGF ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. EGF also interacts with the following gene(s): FLNA. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.