|The Chicken E2 hsd17b1 (Catalog #MBS284852) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS284852 ELISA Kit recognizes Chicken (Gallus, General) E2.
The E2 hsd17b1 product has the following accession number(s) (GI #223718074) (NCBI Accession #NP_000404.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Intended Uses: For the quantitative detection of Chicken Estradiol (E2) concentration in serum and other biological fluids.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: serum and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 40 pg/mL - 1500 pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%. Chicken Estradiol (E2) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Bone, Brain, Embryonic Tissue, Intestine, Mammary Gland, Placenta, Stomach, Testis, Uterus, Vascular tissues are correlated with this protein. E2 also interacts with the following gene(s): CYP19A1, CYP1A1, CYP1B1, CYP3A4, HSD17B2, HSD17B3, HSD17B7, SULT1E1, UGT2B17, UGT2B7. Adenocarcinoma, Breast Neoplasms, Disorders of Sex Development, Endometriosis, Genital Diseases, Male, Liver Diseases, Ovarian Diseases, Prostatic Diseases, Prostatic Neoplasms, Urogenital Abnormalities are some of the diseases may be linked to Chicken Estradiol (E2) ELISA Kit.