|The Rat E1 clf_112599 (Catalog #MBS284474) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS284474 ELISA Kit recognizes Rat (Rattus, General) E1.
The E1 clf_112599 product has the following accession number(s) (GI #358254166) (NCBI Accession #GAA54196.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Estrone (also oestrone) is an estrogenic hormone secreted by the ovary as well as adipose tissue.Estrone is one of the three estrogens, which also include estriol and estradiol. Estrone is the least abundant of the three hormones, estradiol is present almost always in the reproductive female body, and estriol is abundant primarily during pregnancy.
Estrone is relevant to health and disease states because of its conversion to estrone sulfate, a long-lived derivative. Estrone sulfate acts as a reservoir that can be converted as needed to the more active estradiol.
Estrone is synthesized via aromatase from androstenedione, a derivative of progesterone. The conversion consists of the de-methylation of C-19 and the aromaticity of the \'A\' ring. This reaction is similar to the conversion of testosterone to estradiol.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate E1 in samples. An antibody specific for E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for E1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Rat Estrone (E1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.