DS clia kit product blog
Tags: CLIA Kit; Human CLIA Kit; DS (Dermatan Sulfate); DS clia kit;
The Human DS n/a (Catalog #MBS2532530) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2532530 CLIA Kit recognizes Human DS.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human DS (Dermatan Sulfate) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of DS concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to DS. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of DS. You can calculate the concentration of DS in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL.