DKK1 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; DKK1; DKK1 elisa kit; Dickkopf Related Protein 1 (DKK1);
The Human DKK1 dkk1 (Catalog #MBS268956) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS268956 ELISA Kit recognizes Human DKK1.The DKK1 dkk1 product has the following accession number(s) (GI #7110719) (NCBI Accession #NP_036374.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Dickkopf Related Protein 1 (DKK1) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human DKK1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
Samples: Human serum, plasma or cell culture supernatant
Assay Type: Quantitative Sandwich
Detection Range: 10 ng/mL-0.156 ng/mL
Sensitivity: Up to 0.05 ng/mL. Intra-assay Precision: <= 8%
Inter-assay Precision: <= 12%. Human Dickkopf Related Protein 1 (DKK1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Bone Marrow, Brain, Connective Tissue, Embryonic Tissue, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. DKK1 also interacts with the following gene(s): CTNNB1, FZD1, FZD8, KREMEN1, KREMEN2, LRP5, LRP6, RSPO1, WNT1, WNT3A. Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Hematologic Diseases, Inflammation, Lung Diseases, Necrosis, Neoplasm Metastasis, Neoplasms, Nervous System Diseases are some of the diseases may be linked to Human Dickkopf Related Protein 1 (DKK1) ELISA Kit. The following patways have been known to be associated with this gene.