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DHEA-S elisa kit product blog

Posted on 2015-08-08 15:45:21 by mybiosource_staff
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Tags: ELISA Kit; Porcine ELISA Kit; DHEA-S; DHEA-S elisa kit;
The Porcine DHEA-S n/a (Catalog #MBS2503449) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2503449 ELISA Kit recognizes Porcine (Pig, Swine, General) DHEA-S.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

DHEA-S n/a

Samples: Serum, plasma and other biological fluids
Assay Type: Competitive
Detection Range: 31.25-2000ng/mL
Sensitivity: The minimum detectable dose of Porcine DHEA-S is 18.75ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine DHEA-S concentrations in serum, plasma and other biological fluids. Porcine DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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