DF elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Dengue fever antibody; DF elisa kit;
The Human DF df (Catalog #MBS005492) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS005492 ELISA Kit recognizes Human DF.The DF df product has the following accession number(s) (GI #49168634) (NCBI Accession #CAG38812.1) (Uniprot Accession #Q6FHW3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the qualitative determination of activated Human Dengue Fever Antibody (DF) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.
Principle of the Assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human DF in samples. Add sample to wells pre-coated with one Human Dengue Fever antigen, at same time add HRP-conjugated Human Dengue Fever secondy antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human DF in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.
Samples: Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.
Assay Type: Sandwich. Intra-assay Precision: Intra-assay CV (%) are less than 15%.
Inter-assay Precision: Inter-assay CV (?) are less than 15%. Human Dengue Fever Antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Heart, Liver, Lung, Mouth, Muscle, Pharynx, Vascular tissues are correlated with this protein.