|The Mouse DBP/GC n/a (Catalog #MBS766171) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS766171 ELISA Kit recognizes Mouse (Murine, General) DBP/GC.
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(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse DBP/GC (Vitamin D-binding protein) with the following immunoassay(s):
Typical Standard Curve/Testing Data
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DBP/GC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DBP/GC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DBP/GC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DBP/GC can be calculated.
Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 3.9-250ng/ml
Sensitivity: <2.34ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DBP/GC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DBP/GC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Mouse DBP/GC (Vitamin D-binding protein) or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.