Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow DBP/GC elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • SKP2 elisa kit blog
 • HBB elisa kit blog
 • PDAP1 clia kit blog
 • TREM-1 elisa kit blog
 • MHC-III/H-2III elisa kit blog
 • COL10 elisa kit blog
 • PDE1C elisa kit blog
 • AKT1S1 elisa kit blog
 • LDLR elisa kit blog
 • PDIA2 elisa kit blog
 • PS3 elisa kit blog
 • TPM2 elisa kit blog
 • TNR elisa kit blog
 • LDL elisa kit blog
 • HSP72 elisa kit blog
 • ASB6 elisa kit blog
 • ASB elisa kit blog
 • NOX1 elisa kit blog
 • IL-2R elisa kit blog
 • ABHD1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • PICP ELISA Kit
 • OTUB2 cDNA Clone
 • Transforming Growth Factor beta1 (TGFbeta1) ELISA Kit
 • Tissue factor (F3) Antibody
 • fibroblast growth factor 2 (basic) (FGF2) ELISA Kit
 • Chikungunya Virus Antibody
 • Thyroid Hormone Receptor, a2-Isotype (THRA) Antibody
 • Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit
 • RSPO2 Antibody
 • ITGA4 Antibody
 • Clostridium difficile toxin B Antibody
 • glucagon-like peptide-1, GLP-1 ELISA Kit
 • SHMT2 Antibody
 • CASP9 ELISA Kit
 downarrow more ...
separator

DBP/GC elisa kit product blog

separator
Posted on 2017-10-11 07:17:33 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; DBP/GC (Vitamin D-binding protein); DBP/GC elisa kit; DBP/GC;
separator
The Mouse DBP/GC n/a (Catalog #MBS766171) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS766171 ELISA Kit recognizes Mouse (Murine, General) DBP/GC.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse DBP/GC (Vitamin D-binding protein) with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data DBP/GC.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DBP/GC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DBP/GC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DBP/GC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DBP/GC can be calculated.

Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 3.9-250ng/ml
Sensitivity: <2.34ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DBP/GC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DBP/GC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Mouse DBP/GC (Vitamin D-binding protein) or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions