CytoSelect Proliferating Cell Nuclear Antigen (PCNA) elisa kit product blog
Tags: ELISA Kit; CytoSelect Proliferating Cell Nuclear Antigen (PCNA); CytoSelect Proliferating Cell Nuclear Antigen (PCNA) elisa kit;
The CytoSelect Proliferating Cell Nuclear Antigen (PCNA) n/a (Catalog #MBS168132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit with the following immunoassay(s):
Testing Data
Testing Data #2
Background: Proliferating Cell Nuclear Antigen (PCNA) was originally identified as an auxiliary factor for DNA polymerase delta, and has been described as a DNA sliding clamp that acts as a polymerase processivity factor. PCNA aids in processivity through association with various DNA replication associated proteins. In addition to DNA replication, PCNA function is associated with chromatin remodeling, sister-chromatid cohesion and cell cycle control. PCNA is often used as a marker for cell proliferation. In addition to its role in DNA replication and proliferation, PCNA plays a role in DNA repair. PCNA activates the DNA damage tolerance pathway in two ways. First, PCNA promotes translesion synthesis through DNA polymerase zeta (zeta), whereby DNA replication stalled at a damaged site can be essentially bypassed, ultimately leaving a mutation behind for the sake of cell survival. Secondly, the PCNA promotes the "template switch" pathway where the homologous recombination machinery is recruited to bypass DNA damage. PCNA itself is post-translationally modified with ubiquitin which activates one or the other DNA damage tolerance pathway: mono-ubiqutination of lysine 164 activates translesion synthesis, while further polyubiquitination at lysine 63 favors the "template switch" pathway. CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mL PCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.