|The CytoSelect Cell Migration and Invasion Assay (8 um) n/a (Catalog #MBS168493) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
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Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the CytoSelect 96-well Cell Migration and Invasion Assay (8 um), Fluorometric, Combo Kit with the following immunoassay(s):
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Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect 96-well Cell Migration and Invasion Assay Kit utilizes a polycarbonate membrane plate (8 mum pore size) or basement membrane-coated inserts to assay the migratory or invasive properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory or non-invasive cells (i.e. cotton swabbing). Any migratory or invasive cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye. The CytoSelect 96-well Cell Migration and Invasion Assay Kit provides a robust system for the quantitative determination of cell migration. Each assay contains sufficient reagents for the evaluation of 96 samples. The 8 mum pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 mum) is recommended.