CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric) assay kit product blog
Tags: Assay Kit; CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric); CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric) assay kit;
The CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric) n/a (Catalog #MBS169063) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: The CytoSelect Cell Adhesion Assay Kit utilizes a Fibronectin-coated 48-well plate (see Adhesion Plate Layout below). First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are washed away, and the adherent cells are fixed/stained. Finally, the stain is extracted and quantified colorimetrically.
Background: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Human Fibronectin-coated wells, 8 BSA-coated wells).