Cys-C clia kit product blog
Tags: CYS-C; CLIA Kit; Rat CLIA Kit; Cys-C (Cystatin C); Cys-C clia kit;
The Rat Cys-C cst3 (Catalog #MBS2531097) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531097 CLIA Kit recognizes Rat Cys-C.The Cys-C cst3 product has the following accession number(s) (GI #755738) (NCBI Accession #CAA29096.1) (Uniprot Accession #P01034). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Rat Cys-C (Cystatin C) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of PCX concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PCX. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PCX and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PCX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PCX. You can calculate the concentration of PCX in the samples by comparing the RLU value of the samples to the standard curve.
Samples: Serum, Plasma And Other Biological Fluids
Assay Type: Quantitative Sandwich
Detection Range: 31.25-2000 pg/mL
Sensitivity: 18.75pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanPCX were tested 20 times on one plate, respectively
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanPCX were testedon 3 different plates, 20 replicates in each plate. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Vascular tissues are correlated with this protein. Cys-C also interacts with the following gene(s): APOA1, APP, B2M, CTSB, SAA1, TTR.