CTSD assay kit product blog
Tags: Cathepsin D; CTSD; Assay Kit; CTSD assay kit;
The CTSD ctsd (Catalog #MBS841865) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.The CTSD ctsd product has the following accession number(s) (GI #4503143) (NCBI Accession #NP_001900.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Cathepsin D Inhibitor Screening Kit (Fluorometric) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Cathepsin D Inhibitor Screening Kit: Rapid & Convenient Fluorometric Assay to Screen Cathepsin-D Inhibitors. Includes Inhibitor Control, Pepstatin A. Suitable for High-Throughput Screening. 100 Assays.
Background: Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group MCA, which can then easily be measured using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).
Summary: Detection method- Fluorescence (Ex/Em = 328/460 nm)
Kit size- 100 assays
Applications- Assay kit to detect putative Cathepsin D inhibitors.
Samples: Purified Cathepsin D, Recombinant Cathepsin D. Features & Benefits: Simple one-step procedure; takes only 1 hours
Fast, robust and convenient
Cathepsin-D inhibitor screening assay is a fast and robust assay to screen potential inhibitors
Positive control inhibitor, Pepstatin An is included in the kit. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Eye, Kidney, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Fibrosis, Inflammation, Liver Neoplasms, Necrosis, Neoplasm Invasiveness, Nervous System Diseases, Seizures are some of the diseases may be linked to Cathepsin D Inhibitor Screening Kit (Fluorometric). CTSD also interacts with the following gene(s): BID, CTSA, CTSB, CTSS, ESR1, TP53.