Ctcfl elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; CTCFL; CTCFL elisa kit; Transcriptional repressor CTCFL;
The Mouse Ctcfl ctcfl (Catalog #MBS2885194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2885194 ELISA Kit recognizes Mouse Ctcfl.The Ctcfl ctcfl product has the following accession number(s) (GI #124487069) (NCBI Accession #NP_001074856.1) (Uniprot Accession #A2APF3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Transcriptional repressor CTCFL ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm � 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Type: Competitive
Detection Range: 0.625-40 ng/mL
Sensitivity: 0.33 ng/mL. Intra-Assay CV: <=5.9%
Inter-Assay CV: <=9.3%
Recovery: 116%. Mouse Transcriptional repressor CTCFL ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Ctcfl also interacts with the following gene(s): Bag1, Magea1, Myc, Prmt7, Prss50. Skin tissues are correlated with this protein. Breast Neoplasms, Carcinoma, Genital Diseases, Male, Infertility, Male, Lung Diseases, Lung Neoplasms, Neoplasms, Experimental, Ovarian Diseases, Ovarian Neoplasms, Skin Diseases are some of the diseases may be linked to Mouse Transcriptional repressor CTCFL ELISA Kit.